Wednesday, 8th December 49th Annual Scientific Meeting of the Australian and New Zealand Society for Immunology 2021

Title - Advance Immunology Discovery in Spatial Biology with GeoMx DSP
Chairperson - Qianqiao Tang
Speakers -

• Marshall Feterl, Technical Sales Specialist, NanoString

• Antje Blumenthal, University of Queensland

Description - Through NanoString GeoMx® Digital Spatial Profiler (DSP), researchers can map the spatial architecture of a cell and how it talks to and interacts with its surroundings. Spatial biology is like being inside a tissue sample at a molecular level. It enables researchers to see things that are not possible by sequencing or any other technologies out there. With GeoMx® Digital Spatial Profiler (DSP), researchers can profile up to 22,000 RNA or >180 proteins on a single slide. The capability of GeoMx® DSP to work with multiple clinical sample types including FFPE, Fresh Frozen and Tissue Microarray also make it an ideal tool for those who like to explore the wealth of archived samples.

In this industrial breakout session, we will lead you through a journey of Spatial Biology and answer questions to those who are new to this frontier. You will also learn more about how GeoMx DSP can help research to significant breakthrough in immunology.

The symposium will start with Dr. Marshall Feterl, Technical Sales Specialist from NanoString to present on the principles and mechanism of GeoMx DSP. Professor. Antje Blumenthal from University of Queensland Diamantina Institute to present her research work in mycobacterium tuberculosis with GeoMx DSP titled “Spatial exploration of lung pathology in mycobacterium tuberculosis infection”.

• Rapid viral adaptation to the CD8⁺ T-cell immune response during acute HIV infection - Eric Alves
• DNA methyltransferases drive gastric cancer growth and present a therapy target for gastric cancer - Anne Huber
• Early expansion of CD38+ICOS+ GC Tfh cells in draining lymph nodes during human germinal centre response to influenza vaccination - Hannah Law
• Depletion of TVM cells with LCMV infection leads to selective retention of more functional, less senescent cells - Tabinda Hussain
• Exploring natural killer cell immunity during Salmonella infection - Timothy McCulloch
• Specialized gut cells orchestrate the interactions between microbiota and immunity - Wang Cao

• Lipopolysaccharide promotes Drp1-dependent mitochondrial fission and associated inflammatory responses in macrophages - Syeda Farhana Afroz
• Mesenchymal plasticity of Tasmanian devil facial tumour cells during in vivo vaccine and immunotherapy trials - Amanda Patchett
• Investigating the role of type 2 innate lymphoid cells in multiple myeloma - Camille Guillerey
• Phenotypic characterisation of tissue-resident Tregs in human lymphoid tissue and their potential roles in maintaining homeostasis - Rehana Hewavisenti
• Nalfurafine reduces neuroinflammation and drives remyelination in models of CNS demyelinating disease - Lisa Denny
• Influenza, but not SAS-CoV-2, infection induces a rapid interferon response that wanes with age and diminished tissue resident memory CD8+ T cells - Linda Wakim
• Lymphodepleted non-human primate model for the assessment of acute on-target and off-tumor toxicity of human CAR-T cells - Shigeki Yagyu
• Repeated influenza vaccination provides cumulative protection from distinct H3N2 viruses - Sophie Valkenburg

• Post-transplant cyclophosphamide combined with interleukin-6 receptor blockade increases regulatory T cells but does not improve survival compared to cyclophosphamide alone.  - Chloe Sligar
• An anti-human P2X7 monoclonal antibody prevents graft-versus-host disease in humanised mice - Amal Elhage
• Infection-induced WNT responses in macrophages are regulated by innate immune receptor activation - Johanna K Ljungberg
• Combinational therapy with post-transplant cyclophosphamide and the P2X7 antagonist Brilliant Blue-G increases human CD39⁺ regulatory T cells and reduces GVHD in a humanised mouse model of graft-versus-host disease - Peter Cuthbertson
• IFNβ is a potent adjuvant for cancer vaccination strategies - Katherine Audsley
• Dual-specific chimeric antigen receptor T cells eliminate heterogeneous tumours - Hannah E Hughes-Parry
•  Dissecting the mechanisms of type I interferon-driven anti-tumour immunity to enhance cancer control - Hannah V Newnes
• Type I interferon subtypes differentially activate natural killer cell activity against leukaemia - Samantha Barnes
• Transcriptional regulation of tissue resident memory T cells in cancer - Kai Plunkett
• Overexpression of memory associated transcription factors promotes chimeric antigen receptor (CAR) T cell stemness and anti-tumour efficacy - Jack D Chan
• Intracellular iron controls the critical immune regulatory enzyme indoleamine 2,3-dioxygenase 1 (IDO1) in human macrophages  - Bentotage S.M Fernando
• The role of heme metabolism in controlling the central immune regulatory enzyme indoleamine 2,3-dioxygenase 1 (IDO1) in human macrophages - Bentotage S.M Fernando
• The role of cellular energy metabolism in controlling indoleamine 2,3-dioxygenase 1 (IDO1) in human macrophages - Bentotage S.M Fernando
• PD-1 inhibits T cell proliferation by decreasing division destiny - Melissa Butler
• Treatment of cutaneous tumours in mouse pinnae with local dose sparing concentrations of immunotherapeutic antibodies, result in potent anti-tumour responses and induction of long lived, antigen specific, systemic memory. - Quentin Wright
• Developing a mouse model of neuroblastoma for immunotherapy - Allison Wan
• Developing next generation armoured natural killer cells for cancer immunotherapy - Isabella Trew
• Assessing the efficacy of immune agonist immunotherapies in a preclinical model of microsatellite-stable colorectal cancer - Yee Chern Tee
• A delta-inulin adjuvanted SARS-CoV-2 vaccine generates a robust lung-resident cellular and humoral immune response when delivered intratracheally - Erica Stewart
• A 3D bioprinted killing assay to investigate the effect of matrix physical properties on cytotoxic T lymphocyte function - Mir Hadi Seyedzadeh
• CRISPR/Cas9 mediated deletion of the adenosine A2A receptor enhances CAR T cell efficacy - Kevin C Sek
• Harnessing the full potential of T cells for adoptive cell transfer - Terri Scanlon
• Intestinal immune responses to acute infection - Sarah K Sandford
• Direct and indirect effects of cytomegalovirus infection on the graft-versus-leukaemia response - Alda Saldan
• TLR3 ligand poly (I:C) is taken up by tumour-infiltrating macrophages resulting in an IFNa-dependent anti-tumour effect - Francois Rwandamuriye
• Immunoregulatory Role for Complement Receptors in Murine Breast Cancer - Barbara Rolfe
• A cure for food allergy? The immunomodulatory properties of Schistosoma mansoni egg-derived exosomes in food allergy. - Madeleine M Rogers
• Deficiency of γδ T cells promotes lung inflammation following colitis - April L Raftery
• Systematic Analysis of Chemotherapy and Immune Checkpoint Blockade Combinations that Lead to Durable Responses - Nicola Principe
• MAIT cells regulate NK cell mediated tumor immunity - Emma Petley
• Dysfunctional T cell receptor proximal signalling in cancer - Newton Peres
• Characterising the effect of Hck inhibition in a mouse mammary tumour model using single cell sequencing - Michael W Murrey
• Discovery of MR1-expressing cells in vivo - Hamish E G McWilliam
• Investigating the effects of maternal diet on early life immune development - Yan Lu
• Overcoming tumour heterogeneity by engaging host immunity to enhance chimeric antigen receptor T cell therapy of solid cancers - Junyun Lai
• Understanding the relationship between the human gut microbiome and metabolic syndrome - Rina Kumar
• Characterising T-cell migratory cues in glioblastoma lesions - Paris M Kollis
• Development of DC-based immunotherapy to promote anti-tumour CD8 T cell immunity in the liver - Jung Hee Kang
• Uterine natural killer cells are modulated by regulatory T cells to promote uterine spiral artery remodelling in mice - Shanna L Hosking
• Regulatory T cells exhibit phenotypic plasticity prior to inflammation-induced preterm birth in mice - Ella S Green
• Cellular changes in the colonic mucosal immune system in Hirschsprung disease - Ashleigh Gould
• IFN-g signaling is not essential for TCR rearrangement and thymocytes development, but important to suppress T cell lymphoma development - Tingting Ge
• A new role for the adhesion molecule ICAM1 in breast cancer - Anahita Fouladzadeh
• The ratio of exhausted to resident infiltrating lymphocytes is prognostic for colorectal cancer (CRC) patient outcome - Momeneh Foroutan
• CRISPR-mediated PTPN2 deletion in CAR T cells enhances anti-tumour efficacy - Xin Du
• Quantification of bioactive compounds in human milk that may play a role in allergy prevention - Nivedithaa Divakara
• CD137 ligand (4-1BB ligand) mediates immune surveillance of pre-lymphoma B cells - Zhoujie Ding
• Dysregulated T cell memory to intestinal bacterial antigens in human autoimmune and infectious disease: implications for IL-17 inhibitors - Laura Cook
• Immune checkpoint blockade responding tumours get a head start in T cell clonal expansion - Jonathan Chee
• Interleukin-24 involvement in the intestinal response to curdlan in SKG mice - Amy J Cameron
• Vasculogenic mimicry structures in melanoma support the recruitment of pro-tumorigenic monocytes - Claudine Bonder
• Extracellular traps are prevalent in bronchoalveolar lavage from children with non-cystic fibrosis bronchiectasis - Amy S Bleakley
• Pre-clinical validation of a LGR5-targeting CAR-T against colorectal cancer  - Veronika Bandara
• Mucosal protein-based vaccination with a TLR2-stimulating adjuvant induces potent local immunity against SARS-CoV-2 in mice - Anneliese S Ashhurst
• Immunologic parameters for disease activity in patients with rheumatoid arthritis undertherapy of TNF antagonists. - Naael H Ali
• Type 1 conventional dendritic cell fate and function are controlled by DC-SCRIPT - Shengbo Zhang
• PKR is a sensor of proteotoxic stress via accumulation of cytoplasmic IL-24 - Sophia Davidson
• MAIT cells expand in the absence of NKT and γδT cells  - Calvin Xu
• BCG vaccine induces long-term epigenomic remodeling of human monocytes - Boris Novakovic
• Effect of Pathogen Associated Molecular Patterns on the development of Neurogenic Heterotopic Ossifications after Spinal Cord Injuries   - Selwin Samuel
• Fingertip blood samples as a new method to assess lymphocyte diurnal variation - Dayna Bushell
• Impact of COVID-19 and PPE measures on occupational skin disease in healthcare workers - Sumir Chawla
• CD1a-restricted T cells: a subset of “unconventional” T cells like no other - Catriona V Nguyen-Robertson
• Characterizing B lymphocytes in healthy subjects and in subjects with stable coronary artery disease and acute myocardial infarction - Georgina Bird
• Mvk mutant mouse models of mevalonate kinase deficiency reveal a role for increased body temperature and NLRP3 in inflammatory flares - Oliver P Skinner
• Changes in plasma cytokine and circulating immune cells following exercise-induced muscle damage - Odette M Shaw
• Immunogenicity of COVID-19 vaccines in kidney transplant recipients and their close household contacts - Griffith Perkins
• BLNK, CD40 and CD210 expression on discrete IgA+ and IgG+ B cell subsets in MS patients: modulation by alemtuzumab and during relapse - Felix Marsh-Wakefield
• Contribution of TLR2 and cGAS-STING signalling to the innate response against Daptomycin- sensitive or resistant MRSA. - Nazneen Jahan
• Immunophenotyping of peripheral blood mononuclear cells in septic shock patients with high-dimensional flow cytometry analysis reveals two subgroups with differential responses to immunostimulant drugs - Ya Wang
• Interfering with dendritic cell signalling and development - Mariam Bafit
• MAIT cell responses in the colorectal cancer environment - Jason Kelly
• Functional evaluation of common NOD2 gene variants in patients with antibody deficiency and gastrointestinal complications - Ebony G Blight
• Quantifying T-cell responses in complex disorders of immune dysregulation - Anthony Farchione
• Mucosal Associated Invariant T cells in Patients with Chronic Lymphocytic Leukaemia - Louis Perriman
• Targeting inflammasomes in disease – is there a trade-off? - Sabrina Sofia Burgener
• Bisphosphonate drugs that target the skeleton also inhibit the mevalonate pathway in alveolar macrophages and boost immune responses to endotoxin in the lung. - Emma K Fletcher
• Increased basal and induced phosphoinsitol-3-kinase signalling in B- and T-cells of healthy adults carrying the PTPN22 R620W mutations - Emily SJ Edwards
• BRAF inhibitor Dabrafenib enhances dendritic cell activation - Ee Shan Pang
• Post-transplant cyclophosphamide does not impact graft-versus-leukaemia responses in a humanised mouse model - Debbie Watson
• Long-term perturbation of the transcriptome months after SARS-CoV-2 infection - Feargal J Ryan
• Spatio-temporal regulation of toll-like receptor RP105 localisation in macrophages - Carmen Mathmann
• Assessing the nonspecific effects of whole-cell DTP-containing vaccines in murine models of infection and asthma - Natalie Stevens
• sc-RNASeq reveals innate immune responses regulate age-dependent responses to malaria infection - Tinashe G Chabikwa
• Modulation of inflammatory cytokine production through Interleukin-1 receptor associated kinase-3 (IRAK3) and cGMP - Trang Hong Nguyen
• Probing the molecular mechanisms that curb TLR4-driven inflammatory responses - Thomas E Schultz
• Extravascular MSC treatment induces immunoregulatory innate immune cells to dampen inflammation - Di Zheng
• Stochastic clonal regulation of antibody class switching - Miles Horton
• Dendritic cell paralysis contributes to immunosuppression and secondary infections long after severe infection or trauma; investigation on the mechanisms, diagnostic markers and restorative therapies - Mitra Ashayeripanah
• The spleen is indispensable for the immunosuppressive activity of mesenchymal stromal cells in OVA allergic asthma - Tejasvini Bhuvan
• Human CD141 + dendritic cells (cDC1) are impaired in patients with advanced melanoma but can be targeted to enhance anti-PD-1 in a humanized mouse model - Liam O'Brien
• Improving the phenotype of GD2-specific CAR-T cells for the treatment of glioblastoma - Erica Yeo

Sponsored by Pfizer 

• Overactive STAT3 drives aberrant accumulation of autoimmune-associated CD21^low CD23^low B cells - Etienne N Masle-Farquhar
• Conventional type 1 dendritic cells present native antigen to B cells for generation of T-dependent humoral immunity - Thiago M. Steiner
• Tissue-resident immunity is altered after liver transplantation - Amy Prosser
• How does allergen immunotherapy 'switch-off' allergy? - Anya Jones
• Novel EGFRvIII-specific murine CAR T cells completely eliminate glioblastoma tumours and provides scaffold for further genetic engineering - Rebecca C Abbott
• Temporally restricted activation of IFNβ signaling from inflammatory monocytes determines response to immune checkpoint therapy - Rachael M Zemek