The intestines are a major reservoir for immune cells involved in the elimination of infections, such as T cells. Tissue-resident memory T cells (TRM), a subset of T cells, form and permanently reside in non-lymphoid tissues such as the gut post-clearance of infection, providing accelerated protective immunity against secondary infections. However, little is known about the dynamics and roles of T cells in the large intestine compared to the small intestine during immune responses and in the formation of immunological memory. In the small intestinal epithelium, most TRM are CD8+CD103+CD69+ and their formation requires TGF-ß signalling. We used the acute strain of Lymphocytic Choriomeningitis Virus (LCMV) to characterise and compare T cell kinetics and phenotype in the small and large intestines during acute viral infection in a mouse model. After acute infection, few LCMV-specific TRM formed in the large intestine due to differential TGF-ß-regulated responses and downstream expression of markers such as CD69, CD103 and Ly6c. This effect was also observed after an acute bacterial infection with a gut-infecting strain of Listeria monocytogenes. These studies will contribute to a greater understanding of the development of resident T cell memory in different organs, specifically the large intestine, and how this impacts the immune response to viral and bacterial infection.