In light of the current pandemic, our understanding of how the immune system combats viral infection has become more important than ever. Interferons (IFNs) are key mediators of antiviral immunity. While the functions of Type I Interferons have been extensively studied, the newest member of the Interferon family, Interferon-Lambda (IFN-λ), is yet to be fully investigated. Currently, it is believed that the canonical signalling pathway of the IFN-λ receptor (R, defined as IFN-λR1 and IL-10Rb) is shared with Type I IFN-αR signalling and leads to the transcription of Interferon Stimulated Genes (ISGs). The transcription of these ISGs lead to the production of products that are integral to the immune system’s anti-viral response. However, there is a growing body of evidence that indicates that IFN-λR1 is involved in signalling pathways that differ to IFN-αR. Building on novel preliminary data, this project seeks to differentiate IFN-λR1 signalling from this canonical interferon pathway. Dendritic Cells (DCs), key mediators linking innate and adaptive immunity, are the focus of this project as they are major producers of IFN-λ and are also one of the few haematopoietic cells which express IFN-λR1. While the impact of Type I IFNs on end-stage DC maturation has been well established, little is known about how interferons influence the earlier stages of DC development and whether or not IFN-λ and IFN-α play different roles in this context. Using DCs, the research presented here will compare and contrast the influence IFN-λ and IFN-α have on DC signalling pathways during their development and how this may affect DC function after activation.